TY  - JOUR
TI  - A role for Dimethylarginine Dimethylaminohydrolase 1 (DDAH1) in mammalian development 
AU  - Breckenridge, RossA.
AU  - Kelly, Peter
AU  - Nandi, Manasi
AU  - Vallance, PatrickJ.
AU  - Ohun, TimothyJ.
AU  - Leiper, James
T2  - The International Journal of Developmental Biology
AB  - Nitric oxide has been linked to a number of embryonic processes, yet the role of nitric oxide signalling in development remains largely unknown. Dimethylarginine Dimethylaminohydrolase 1 and 2 (DDAH1/2) catalyse the breakdown of asymmetric dimethylarginine, an endogenous inhibitor of nitric oxide production, and may also have nitric oxide-independent functions. We have generated transgenic mice targeting the DDAH1 and DDAH2 loci. Here we report that homozygous DDAH1 null embryos are generated at low frequency, and do not progress through embryonic development. During normal development DDAH1 RNA is expressed in the left ventricle, cardiac outflow tract and developing vasculature. In contrast, DDAH2 homozygous null mice are viable and fertile, with a normal lifespan. DDAH2 expression is seen in the developing left ventricle and cardiac outflow tract, and additionally in the peripheral nervous system. Both DDAH1 and 2 are expressed in the developing limb buds in patterns overlapping areas with high nitric oxide synthase activity. These expression patterns implicate DDAH1 and DDAH2 in embryonic development, possibly through specific effects on nitric oxide pathways.
PY  - 2010
DO  - 10.1387/ijdb.072356rb
VL  - 54
IS  - 1
SP  - 215
EP  - 220
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/072356rb
Y2  - 2024/11/05/11:25:12
ER  -