TY  - JOUR
TI  - An efficient method for isolation of murine bone marrow mesenchymal stem cells
AU  - Nadri, Samad
AU  - Soleimani, Masoud
AU  - Hosseni, RezaH.
AU  - Massumi, Mohammad
AU  - Atashi, Amir
AU  - Izadpanah, Reza
T2  - The International Journal of Developmental Biology
AB  - Mesenchymal stem cells (MSCs) have been isolated based on the ability of adherence to plastic surfaces. The potential of these cells to differentiate along multiple lineages is the key to identifying stem cell populations in the absence of molecular markers. Here we describe a homogenous population of MSCs from mouse bone marrow isolated using a relatively straightforward and novel approach. This method is based on the combination of frequent medium change (FMC) and treatment of the primary cultures with trypsin. Cells isolated using this method demonstrated the MSCs characteristics including their ability to differentiate into mesenchymal lineages. MSCs retained the differentiation potentials in expanded cultures up to 10 passages. Isolated MSCs were reactive to the CD44, Sca-1, and CD90 cell surface markers. MSCs were negative for the hematopoietic surface markers such as CD34, CD11b, CD45, CD31, CD106, CD117 and CD135. The data presented in this report indicated that this method can result in efficient isolation of homogenous populations of MSCs from mouse bone marrow.
PY  - 2007
DO  - 10.1387/ijdb.072352ns
VL  - 51
IS  - 8
SP  - 723
EP  - 729
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/072352ns
Y2  - 2024/11/21/22:21:31
ER  -