TY  - JOUR
TI  - Zebrafish <I>spata2</I> is expressed at early developmental stages
AU  - Moro, Enrico
AU  - Maran, Claudio
AU  - Slongo, M. Liliana
AU  - Argenton, Francesco
AU  - Toppo, Stefano
AU  - Onisto, Maurizio
T2  - The International Journal of Developmental Biology
AB  -  Spata2 (spermatogenesis-associated protein 2) was originally described as a novel gene involved in the spermatogenic process. In this study, we cloned a potential zebrafish spata2 orthologue. The consensus open reading frame (1650 bp) encodes a polypeptide of 550 amino acids which shares 37% identity with the human SPATA2. Bioinformatic analysis reveals a small pattern PW [KR] KE [YF][RK] which seems to be of particular interest in the light of its strong conservation between SPATA2 and the recently discovered TAMO protein of D. melanogaster. RT-PCR analysis in adult zebrafish tissues revealed that spata2 mRNA has a broad distribution. Whole-mount in situ hybridization demonstrated that spata2 transcripts are maternally derived and becomes strongly localized in the central nervous system at early developmental stages. From 5 dpf, spata2 expression becomes detectable in the gut and pronephric duct epithelium, suggesting a wide tissue function during vertebrate development.
PY  - 2007
DO  - 10.1387/ijdb.062220em
VL  - 51
IS  - 3
SP  - 241
EP  - 246
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/062220em
Y2  - 2024/11/05/17:17:17
ER  -