TY  - JOUR
TI  - Enhanced development of porcine embryos cloned from bone marrow mesenchymal stem cells
AU  - Jin, Hai-feng
AU  - Kumar, B Mohana
AU  - Kim, Jung-Gon
AU  - Song, Hye-Jin
AU  - Jeong, Yeon-Ji
AU  - Cho, Seong-Keun
AU  - Balasubramanian, Sivasankaran
AU  - Choe, Sang-Yong
AU  - Rho, Gyu-Jin
T2  - The International Journal of Developmental Biology
AB  - In the present study, we have characterized an isolated population of porcine bone marrow mesenchymal stem cells (MSCs) for multilineage commitment and compared the developmental potential of cloned embryos with porcine MSCs and fetal fibroblasts (FFs). MSCs exhibited robust alkaline phosphatase activity and later transformed into mineralized nodules following osteoinduction. Furthermore, MSCs underwent adipogenic and chondrogenic differentiation by producing lipid droplets and proteoglycans, respectively. Primary cultures of FFs from a female fetus at ~30 day of gestation were established. Donor cells at 3-4 passage were employed for nuclear transfer (NT). Cell cycle analysis showed that the majority of MSCs in confluence were in the G0/G1 stage. Cumulus-oocyte complexes were matured and fertilized in vitro (IVF) as control. The cleavage rate was significantly (P
PY  - 2007
DO  - 10.1387/ijdb.062165hj
VL  - 51
IS  - 1
SP  - 85
EP  - 90
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/062165hj
Y2  - 2024/11/05/15:24:12
ER  -