TY - JOUR TI - Developmental expression of Shisa-2 in Xenopus laevis AU - Silva, Ana-Cristina AU - Filipe, Mario AU - Vitorino, Marta AU - Steinbeisser, Herbert AU - Belo, Jose-Antonio T2 - The International Journal of Developmental Biology AB - Shisa is an antagonist of Wnt and FGF signaling, that functions cell autonomously in the endoplasmic reticulum (ER) to inhibit the post-translational maturation of Wnt and FGF receptors. In this paper we report the isolation of a second Xenopus shisa gene (Xshisa-2). Xenopus Shisa-2 shows 30.7% identity to Xshisa. RT-PCR analysis indicated that Xshisa-2 mRNA is present throughout early development and shows an increased expression during neurula and tailbud stages. At neurula stages Xenopus shisa-2 is initially expressed in the presomitic paraxial mesoderm and later in the developing somites. The expression profiles and pattern of Xshisa and Xshisa-2 differ significantly. During gastrulation only Xshisa mRNA is present in the Spemann-Mangold organizer and later on becomes restricted to the neuroectoderm and the prechordal plate. PY - 2006 DO - 10.1387/ijdb.062141as VL - 50 IS - 6 SP - 575 EP - 579 J2 - Int. J. Dev. Biol. LA - en SN - 0214-6282 SN - 1696-3547 UR - https://ijdb.ehu.eus/article/062141as Y2 - 2024/11/05/15:23:53 ER -