TY  - JOUR
TI  - Metamorphosis of <I>Hydractinia echinata</I> (Cnidaria) is caspase-dependent
AU  - Seipp, Stefanie
AU  - Wittig, Karola
AU  - Stiening, Beate
AU  - Bottger, Angelika
AU  - Leitz, Thomas
T2  - The International Journal of Developmental Biology
AB  - Apoptotic cell death plays an important role in many developmental pathways in multicellular animals. Here, we show that metamorphosis in the basal invertebrate Hydractinia echinata (Cnidaria) depends on the activity of caspases, the central enzymes in apoptosis. Caspases are activated during metamorphosis and this activity can be measured with caspase-3 specific fluorogenic substrates. In affinity labelling experiments 23/25 kDa bands were obtained, which represented active caspase. Specific inhibition of caspase activity with caspase-3 inhibitors abolished metamorphosis completely, reversibly and in a dose-dependent manner. This suggests that caspase activity is indispensable for metamorphosis in Hydractinia echinata.
PY  - 2006
DO  - 10.1387/ijdb.052012ss
VL  - 50
IS  - 1
SP  - 63
EP  - 70
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/052012ss
Y2  - 2024/11/24/06:04:52
ER  -