TY  - JOUR
TI  - Two members of the <I>Fxr</I>  gene family, <I>Fmr1 </I> and <I>Fxr1</I>, are differentially expressed in <I>Xenopus tropicalis</I>
AU  - Blonden, Lau
AU  - van 't Padje, Sandra
AU  - Severijnen, Lies-anne
AU  - Destree, Olivier
AU  - Oostra, BenA.
AU  - Willemsen, Rob
T2  - The International Journal of Developmental Biology
AB  - The Fxr gene family is composed of three members, FMR1, FXR1  and FXR2. The FMR1  gene is involved in the fragile X syndrome, whereas for the other two members, no human disorder has been identified yet. An appropriate animal model to study in vivo  gene function is essential to unravel the cellular function of the gene products FMRP, FXR1P and FXR2P, respectively. In Xenopus tropicalis  both Fmr1  and Fxr1  were identified; however, unexpectedly Fxr2  was not. Here we describe the characterization of both Fmrp and Fxr1p in Xenopus tropicalis. Fmrp is expressed ubiquitously throughout the embryo during embryonic development, whereas Fxr1p shows a more tissue-specific expression particularly during late embryonic development. In adult frogs both proteins are highly expressed in most neurons of the central nervous system and in all spermatogenic cells in the testis. In addition, Fxr1p is also highly expressed in striated muscle tissue. Western blotting experiments revealed only one prominent isoform for both proteins using different tissue homogenates from adult frogs. Thus, for in vivo  gene function studies, this relative simple animal model may serve as a highly advantageous and complementary model.
PY  - 2005
DO  - 10.1387/ijdb.051974lb
VL  - 49
IS  - 4
SP  - 437
EP  - 441
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/051974lb
Y2  - 2024/11/05/17:18:04
ER  -