TY  - JOUR
TI  - The generation of insulin-producing cells from embryonic stem cells - a discussion of controversial findings 
AU  - Kania, Gabriela
AU  - Blyszczuk, Przemyslaw
AU  - Wobus, AnnaM.
T2  - The International Journal of Developmental Biology
AB  -  The derivation of insulin-producing cells from embryonic stem (ES) cells has been controversially described. Whereas several authors showed successful differentiation of mouse ES cells into islet-like clusters, others could not confirm the results. Here, we present a detailed comparison of the various strategies used to generate pancreatic cells with respect to protocols and differentiation factors and give an explanation of the contradictory findings. It is suggested that the selection or enrichment of ES-derived nestin-positive cells should be avoided, since these cells are already committed to a neural fate before pancreatic differentiation is induced. 
PY  - 2004
DO  - 10.1387/ijdb.041925gk
VL  - 48
IS  - 10
SP  - 1061
EP  - 1064
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/041925gk
Y2  - 2024/11/05/17:20:50
ER  -