TY  - JOUR
TI  - Embryonic stem cells differentiate into insulin-producing cells without selection of nestin-expressing cells 
AU  - Blyszczuk, Przemyslaw
AU  - Asbrand, Christian
AU  - Rozzo, Aldo
AU  - Kania, Gabriela
AU  - St-onge, Luc
AU  - Rupnik, Marjan
AU  - Wobus, AnnaM.
T2  - The International Journal of Developmental Biology
AB  -  We present a new strategy for the differentiation of embryonic stem (ES) cells into insulin-producing cells via a multi-step process without selection and induction of nestin-positive cells. During ES cell differentiation, transcript levels of genes characteristic of early and mature beta cells including Pdx1, Pax4, insulin and islet amyloid pancreatic peptide are up regulated. Islet-like clusters are characterized by expression of C-peptide, insulin and partially cytokeratin 19 as well as by ion channel activity similar to that found in embryonic beta cells. Cells of islet-like clusters show glucose-dependent insulin release at terminal stage. At an intermediate stage, nestin is partially co-expressed with C-peptide and cytokeratin 19, whereas islet-like clusters at the terminal stage are nestin-negative. We conclude that expression of nestin and cytokeratin 19 is a normal property of ES cells preceding differentiation into C-peptide/insulin-producing cells without any selection for nestin-positive phenotypes. 
PY  - 2004
DO  - 10.1387/ijdb.041904pb
VL  - 48
IS  - 10
SP  - 1095
EP  - 1104
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/041904pb
Y2  - 2024/12/22/18:07:20
ER  -