TY  - JOUR
TI  - Developing chick embryos express a protein which 
shares homology with the nuclear pore complex protein Nup88 present in human tumors
AU  - Schneider, José
AU  - Linares, Rafael
AU  - Martínez-Arribas, Fernando
AU  - Moragues, María-dolores
AU  - Núñez-Villar, María-José
AU  - Palomar, María-angustias
AU  - Pontón, José
T2  - The International Journal of Developmental Biology
AB  - Nup88 is a nuclear pore complex protein which is overexpressed in a variety of 
human tumors of the stomach, colon, liver, pancreas, breast, lung, ovary, uterus, prostate and kidney. 
A monoclonal antibody crossreacting with the yeast 
Candida albicans and Nup88 was used to investigate the expression of cross-reactive antigens in chick embryos, in an attempt to identify 
an experimental model for studying the role played by Nup88 during cell development and 
differentiation. All cells in the trilaminar embryo were labeled with the antibody, but as 
development advanced and organogenesis was completed, expression of the corresponding antigen 
became more restricted. Thus, some structures continued to be intensely labeled (skin epithelium, 
oropharyngeal endothelium, perichondral mesenchymal tissue), whereas others ( muscular tissue, 
vascular endothelium, respiratory endothelium, digestive tract mucosa, peripheral nerves, 
medullary white matter and the retinal axons) were more moderately stained. No immunoreactivity 
was observed in the medullary grey matter or cartilage. A specific band of 53 kDa observed by 
Western blotting of chick embryo extracts suggested that the chicken antigen recognized by the 
monoclonal antibody is the homologue of human Nup88, which is associated with the high proliferation and 
low differentiation of tumor cells. The present results indicate that the role of Nup88 in cell 
differentiation and organ development could be fruitfully investigated using the developing chick embryo 
as an experimental model.
PY  - 2004
DO  - 10.1387/ijdb.031748js
VL  - 48
IS  - 4
SP  - 339
EP  - 342
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/031748js
Y2  - 2024/11/05/15:27:04
ER  -