TY  - JOUR
TI  - Immortalization of type A spermatogonia using the SV40 large T antigen
AU  - Vecino, P
AU  - Uranga, JA
AU  - Alonso, A
AU  - Arechaga, J
T2  - The International Journal of Developmental Biology
AB  - The study of the in vitro behaviour and experimental manipulation of spermatogonia. is limited due to the absence of an effective protocol for long culture of germ stem cells (Nagano et al., 1998). Some years ago, Hofmann et al. (1992) described the possibility of inducing immortalization of germ cells by transfection of the SV40 large T antigen. Nevertheless, these authors only produced continuous type B spermatogonia. cell lines and other somatic cells of the testis. In this communication we describe a modification of their method that can be used to generate continuous cell lines of the most undifferentiated germ cells of the testis, that is type A spermatogonia.
PY  - 2001
VL  - 45
IS  - S1
SP  - S161
EP  - S162
J2  - Int. J. Dev. Biol.
LA  - en
SN  - 0214-6282
SN  - 1696-3547
UR  - https://ijdb.ehu.eus/article/01450161
Y2  - 2024/05/19/14:04:00
ER  -